Experimental Parameters and Properties for the Induction and Maintenance of Plastic Modifications

Inc. Delay: incubation delay. Inc. T, Rec T: incubation and recording temperatures in Celsius degrees. Ext. Pre., Freq. Pre., N Pre., Eff. Dur. Pre.: temporal extension, frequency (Hz), number, and effective duration of presynaptic stimuli. Post.: postsynaptic stimulation characteristics. The effective duration of presynaptic stimulation was estimated as the product of the number of presynaptic stimulations by the duration of excitation per unitary stimulus. This duration was estimated to be 0.01 s, the typical time constant of excitatory postsynaptic potentials (EPSPs). This calculation is valid for stimulation frequencies up to the order of 100 Hz. For higher frequencies, successive EPSPs merge within a train, and the duration of the excitation provoked by an individual train was estimated as the sum of the duration of the train (0.02 s for [58�C60]; 0.035 s for [8,61]) plus the time constant of an EPSP. Maintenance: duration of the monitored maintenance or time constant (�� symbol) of activity-dependent modifications. (50 KB XLS)Inc. Delay：孵化延迟（incubation delay）。Inc. T、Rec T：培养温度与记录温度，单位为摄氏度。Ext. Pre.、Freq. Pre.、N Pre.、Eff. Dur. Pre.：分别指代突触前刺激的时间范围、频率（单位：Hz）、刺激数量与有效时长。Post.：突触后刺激特性。 突触前刺激的有效时长通过突触前刺激总次数乘以单次刺激的兴奋时长计算得到。经估算，单次兴奋时长为0.01秒，即兴奋性突触后电位（EPSPs）的典型时间常数。该计算方法适用于频率不超过100赫兹量级的刺激。对于更高频率的刺激，连续的兴奋性突触后电位会在一串刺激序列中发生融合，此时单串刺激引发的兴奋时长经估算为单串刺激的持续时长（[58–60]对应0.02秒；[8,61]对应0.035秒）加上单个兴奋性突触后电位的时间常数。 Maintenance：维持时长，指所监测的维持时长，或活动依赖性修饰的时间常数（符号τ）。（50 KB XLS）