Alysiella filiformis DSM 16848 RNA-Seq

mRNA-Seq for differential gene expression analysis and delineation of chromosome interaction domains 3 conditions in biological triplicates (exponential growth (OD600 = 0.2) in mod. PY-medium ("B") and BSTSY+FBS ("Serum"), stationary growth (OD600 = 1) in mod. PY-medium ("B") and BSTSY+FBS ("Serum"), growth on solid medium for 24h, exponential growth (OD600 = 0.2) in mod. PY-medium + 25 ug/ml rifampicin for 30 min)

用于差异基因表达分析与染色体互作结构域（chromosome interaction domains）划定的mRNA测序（mRNA-Seq）数据集，共设置3种实验条件，每种条件均设置生物学重复三次。具体培养方案如下：于光密度600（OD600）=0.2的指数生长期，分别在改良PY培养基（mod. PY-medium，标注为"B"）、添加胎牛血清（FBS）的BSTSY培养基（标注为"血清"组）中培养；于OD600=1的稳定生长期，分别在改良PY培养基（标注为"B"）、添加FBS的BSTSY培养基（标注为"血清"组）中培养；在固体培养基上培养24小时；以及在OD600=0.2的指数生长期，于添加25 μg/ml利福平（rifampicin）的改良PY培养基中培养30分钟。