Plasma miRNAs expression in acute ischemic stroke patients with or without recombinant tissue plasminogen activator treatment.

To determine the expression of circulating miRNAs in AIS patients with thrombolysis and without thrombolysis. Grant ID: 81100882 Grant title: The relation between transcription of miR-497 and neurons apoptosis after ischemic stroke Funding source: Chinese National Natural Science Foundation Grantee First Name: Zhen Grantee Last Name:Deng Fifteen AIS patients with thrombolysis treatment and fifteen AIS patients (admission after 8h of onset) without thrombolysis were collected blood sample on the onset of 24h. Fifteen age-match healthy person were as control group. We used miRNA microarrays to detect plasma miRNA expression profiles and real-time PCR verified miRNA expression. Totally, there 3 sample in each group.

本研究旨在明确接受溶栓治疗与未接受溶栓治疗的急性缺血性脑卒中（Acute Ischemic Stroke, AIS）患者外周循环微小核糖核酸（microRNAs, miRNAs）的表达特征。项目编号：81100882；项目名称：缺血性脑卒中后miR-497转录与神经元凋亡的关联关系；资助来源：中国国家自然科学基金；受资助者：邓震（Zhen Deng）。本研究纳入15例接受溶栓治疗的AIS患者，以及15例于发病后8小时入院且未接受溶栓治疗的AIS患者，于发病后24小时采集其外周血样本；另纳入15名年龄匹配的健康志愿者作为正常对照组。本研究采用miRNA微阵列技术检测血浆miRNA表达谱，并通过实时荧光定量PCR（real-time PCR）验证目标miRNA的表达水平。本研究共设置3组，每组3例样本。