Pilot_sequencing_of_Giardia_and_Cryptosporidium_diversity. Pilot_sequencing_of_Giardia_and_Cryptosporidium_diversity

Cryptosporidium and Giardia are now recognised as important enteric eukaryotic parasites, and recent epidemiological studies have implicated them as among the most numerically important causes of childhood moderate and severe diarrhoea globally. Here we are performing a pilot sequencing diverse isolates from both groups. The aim is to study the efficency of sequencing directly from spores isolated from faecal samples and to establish how well reads from different species/assemblages map to the available high-quality reference genomes (for C. parvum, C. hominis, and three Giardia duodenalis assemblages), and whether SNPs can be reliably called across most of the genome. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/隐孢子虫（Cryptosporidium）与贾第鞭毛虫（Giardia）现已被学界认定为重要的肠道寄生真核生物。近期的流行病学研究显示，二者是全球范围内引发儿童中度及重度腹泻的最主要致病原之一。本研究针对这两类寄生虫的多种分离株开展先导测序试验，旨在探究直接从粪便样本分离的孢子的测序效率，并评估不同物种/类群的测序读段（reads）与现有高质量参考基因组（涵盖微小隐孢子虫C. parvum、人隐孢子虫C. hominis，以及3种十二指肠贾第鞭毛虫类群）的比对匹配程度，同时验证能否在基因组的大部分区域可靠检出单核苷酸多态性（Single Nucleotide Polymorphism, SNPs）。本数据集属于预发布数据。有关威康桑格研究所（Wellcome Trust Sanger Institute）共享的预发布数据的规范使用要求（含各类出版暂缓政策的详细说明），请访问 http://www.sanger.ac.uk/datasharing/ 查阅。