AR network in endometrioid carcinoma

AR expression and serum DHT in endometrioid carcinoma were correlated with disease-free survival. To understand the downstream of AR which contributes to poor out come in endometrioid carcinoma, we conducted AR-ChIP sequencing analysis in HEC50B cell lines. Exogenous Flag-tagged AR was overexpressed in HEC50B cells and chromatin IP was performed using Flag M2 antibody.

子宫内膜样癌（endometrioid carcinoma）组织中，雄激素受体（Androgen Receptor, AR）的表达水平及血清二氢睾酮（Dihydrotestosterone, DHT）浓度均与患者无病生存期（disease-free survival）显著相关。为阐明子宫内膜样癌中介导雄激素受体不良预后效应的下游调控机制，我们在HEC50B细胞系中开展了AR靶向染色质免疫共沉淀测序（AR-ChIP sequencing）分析。具体实验操作如下：将外源性Flag标签修饰的雄激素受体在HEC50B细胞中过表达，并利用Flag M2抗体完成染色质免疫共沉淀实验。