Supplementary file 1_Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo.docx

Stable vitiligo significantly impacts patients’ quality of life and presents a considerable challenge to healthcare providers. In recent years, cell therapy has emerged as a promising treatment for stable vitiligo, which is demonstrated encouraging results. Among current cell-based therapies, autologous epidermal cell transplantation is regarded as a safe and cost-effective strategy. However, the therapeutic outcome critically depends on the retention and viability of the transplanted cells at the target site. Therefore, there is an urgent need to develop novel strategies to improve cell retention and maintain cell viability for improving therapeutic efficacy. In this work, a novel cell extraction method was first developed with deal with 2 h at 37°C to obtain epidermal cells while maintaining high cell viability. Subsequently, the crosslinked hyaluronic acid (HA) by BDDE was utilized as 3D scaffold for cell delivery to treat stable vitiligo. By combining the new extraction method with the HA-based hydrogel scaffold, we achieved prolonged cell retention without compromising cell viability. This approach provides a promising, time-saving strategy for treating stable vitiligo using autologous epidermal cells.

稳定型白癜风可显著影响患者的生活质量，同时也为医疗服务提供者带来了不小的诊疗挑战。近年来，细胞疗法已成为稳定型白癜风颇具前景的治疗手段，并已展现出令人鼓舞的治疗效果。在当前各类基于细胞的治疗方案中，自体表皮细胞移植术（autologous epidermal cell transplantation）被认为是一种安全且具有成本效益的策略。然而，治疗效果在很大程度上取决于移植细胞在靶部位的留存率与细胞活性。因此，亟需开发全新的治疗策略，以提升移植细胞的留存率并维持其活性，从而改善治疗效果。在本研究中，我们首先开发了一种新型细胞提取方法：将样本置于37℃下处理2小时以获取表皮细胞，同时保持较高的细胞活性。随后，我们采用经BDDE交联的透明质酸（hyaluronic acid, HA）作为3D细胞递送支架，用于治疗稳定型白癜风。通过将新型细胞提取方法与基于透明质酸的水凝胶支架相结合，我们实现了延长细胞留存时间且不降低细胞活性的目标。该方案为利用自体表皮细胞治疗稳定型白癜风提供了一种颇具前景且省时的治疗策略。