Data from: Transcriptomic analysis reveals pro-inflammatory signatures associated with acute myeloid leukemia progression

<b>Data Set Description</b><b><br></b>

These data are
collected from a total of 70 participants (47 adult; 23 pediatric), all of
which had relapsed or primary resistant acute myeloid leukemia. The data, which
here are separated into an adult and a pediatric dataset, were generated as
part of a study by Stratmann et. al.
(https://doi.org/10.1182/bloodadvances.2021004962). The Stratmann et. al. study
is currently pre-published here: https://ashpublications.org/bloodadvances/article/doi/10.1182/bloodadvances.2021004962/477210/Transcriptomic-analysis-reveals-pro-inflammatory Please note that
separate applications are necessary for the adult and pediatric dataset,
respectively. When applying for access, please indicate which of the datasets
that the application applies for. The adult dataset
contains transcriptome sequencing (RNA-seq) data from 25 diagnosis (D), 45
relapse (R1/R2/R3) and five (5) primary resistant (PR) leukemic samples from 47
patients, as well as five (5) normal CD34+ bone marrow control samples. The pediatric
dataset contains RNA-seq data from 18 diagnosis (D), 22 relapse (R1/R2), six
(6) persistent relapse (R1/2-P) and one (1) primary resistant (PR) leukemic
samples from 23 patients, as well as five (5) normal CD34+ bone marrow control
samples. The leukemic
samples originate from bone marrow or peripheral blood. The normal RNA samples
originate from purified CD34+ bone marrow cells from five different healthy
individuals. Further details regarding the samples are available in the
Supplemental Information part of Stratmann et. al.
(https://doi.org/10.1182/bloodadvances.2021004962). RNA-seq libraries
and associated next-generation sequencing were carried out by the SNP&amp;SEQ
Technology platform, SciLifeLab, National Genomics Infrastructure Uppsala,
Sweden. Libraries were prepared using the TruSeq stranded total RNA library
preparation kit with ribosomal depletion by RiboZero Gold (Illumina). Sequencing
of adult samples was carried out on the Illumina HiSeq2500 platform, generating
paired-end 125bp reads using v4 sequencing chemistry. Sequencing of pediatric
samples was carried out on the Illumina NovaSeq6000 platform (S2 flowcell),
generating paired-end 100bp reads using the v1 sequencing chemistry. The CD34+
bone marrow control samples were sequenced using both platforms (Illumina
HiSeq2500 and NovaSeq6000). Further, all of
these acute myeloid leukemia samples have also been characterized by whole genome
sequencing or whole exome sequencing, with the datasets available under
controlled access through doi.org/10.17044/scilifelab.12292778. <b>Terms for access</b>The adult and
pediatric datasets are only to be used for research that is seeking to advance
the understanding of the influence of genetic and transcriptomic factors on
human acute myeloid leukemia etiology and biology. Use of the
protected pediatric dataset is only for research projects that can merely be
conducted using pediatric acute myeloid leukemia data, and for which the
research objectives cannot be accomplished using data from adults. Applications
intending various method development would thus not be considered as acceptable
for use of the pediatric dataset. Further, the pediatric dataset may not be
used for research investigating predisposition for acute myeloid leukemia based
on germline variants.

For conditional
access to the adult and/or pediatric dataset in this publication, please
contact [e-mail]

<b>数据集描述</b><br><br>本数据集共纳入70名受试者（47名成人、23名儿童），所有受试者均患有复发或原发耐药性急性髓系白血病。本数据集分为成人数据集与儿童数据集，由Stratmann等人的研究产出（DOI：10.1182/bloodadvances.2021004962），该研究目前已预发表：https://ashpublications.org/bloodadvances/article/doi/10.1182/bloodadvances.2021004962/477210/Transcriptomic-analysis-reveals-pro-inflammatory。请注意，成人数据集与儿童数据集需分别申请访问权限，提交申请时请注明所申请的数据集类型。<br><br>成人数据集包含47名患者的25份诊断期（D）、45份复发期（R1/R2/R3）、5份原发耐药（PR）白血病样本，以及5份正常CD34+骨髓对照样本。儿童数据集包含23名患者的18份诊断期（D）、22份复发期（R1/R2）、6份持续复发（R1/2-P）、1份原发耐药（PR）白血病样本，以及5份正常CD34+骨髓对照样本。<br><br>白血病样本来源于骨髓或外周血，正常RNA样本来源于5名健康个体纯化的CD34+骨髓细胞。样本的更多细节可参见Stratmann等人研究的补充材料（https://doi.org/10.1182/bloodadvances.2021004962）。<br><br>RNA测序（RNA-seq）文库及相关下一代测序由瑞典乌普萨拉国家基因组基础设施SciLifeLab的SNP&SEQ技术平台完成。文库制备采用TruSeq链特异性总RNA文库制备试剂盒，并通过RiboZero Gold（Illumina）进行核糖体RNA去除。成人样本的测序使用Illumina HiSeq2500平台，采用v4测序化学试剂，生成125bp双端读长序列；儿童样本的测序使用Illumina NovaSeq6000平台（S2流动槽），采用v1测序化学试剂，生成100bp双端读长序列。CD34+骨髓对照样本同时使用两种平台（Illumina HiSeq2500与NovaSeq6000）进行测序。<br><br>此外，所有急性髓系白血病样本均已通过全基因组测序或全外显子组测序完成表征，相关数据集可通过doi.org/10.17044/scilifelab.12292778申请受控访问。<br><br><b>使用条款</b><br>成人与儿童数据集仅可用于旨在加深理解遗传与转录组学因素对人类急性髓系白血病病因及生物学特性影响的研究。受保护的儿童数据集仅可用于仅能通过儿童急性髓系白血病数据开展、且无法使用成人数据完成研究目标的项目。因此，旨在开展各类方法学开发的申请将不被视为可使用儿童数据集的合理申请。此外，儿童数据集不得用于基于生殖系变异开展的急性髓系白血病易感性相关研究。<br><br>如需获取本出版物中成人和/或儿童数据集的受控访问权限，请联系[e-mail]