Pastinaca sativa P450s - CYP71AJ4 variants in New Zealand and North America

Nucleotide sequences from wild parsnip CYP71AJ4 (angelic in synthase. Genbank EF191021) were obtained by Sanger sequencing. Seeds from individual plants from different populations were harvested to obtain corresponding cDNA. The cDNA was cloned and directly sequenced. Aminoacid translations were obtained using standard codon usage. Alignments of CYP71AJ4 sequences (involved in angular furanocoumarin biosynthesis) with as the reference sequence. Consistent amino acid variabilities were found between some populations. The relationship between sequencing variability and selective pressure is not yet known.

本研究通过桑格测序（Sanger sequencing）获取了野生欧防风CYP71AJ4（当归素合酶，GenBank登录号EF191021）的核苷酸序列。采集不同种群的单株植物种子以获得对应的互补脱氧核糖核酸（cDNA），随后对cDNA进行克隆并直接测序。采用标准密码子使用规则推导得到氨基酸序列。对参与角型呋喃香豆素生物合成的CYP71AJ4序列与参考序列进行比对分析，在部分种群间检测到一致的氨基酸变异位点。目前，测序变异与选择压力之间的关联尚未明确。