Homeostatic control of deep sleep and molecular correlates of sleep pressure in Drosophila

Homeostatic control of sleep is typically addressed through mechanical stimulation-induced forced wakefulness and the measurement of subsequent increases in sleep. A major confound attends this approach: biological responses to deprivation may reflect a direct response to the mechanical insult rather than to the loss of sleep. Similar confounds accompany all forms of sleep deprivation and represent a major challenge to the field. Here we describe a new paradigm for sleep deprivation in Drosophila that fully accounts for sleep-independent effects. Our results reveal that deep sleep states are the primary target of homeostatic control and establish the presence of multi-cycle sleep rebound following deprivation. Furthermore, we establish that specific deprivation of deep sleep state results in state-specific homeostatic rebound. Finally, by accounting for the molecular effects of mechanical stimulation during deprivation experiments, we show that serotonin levels track sleep pressure in t..., All behavioral data were collected using either the Drosophila Activity Monitors or the Ethoscope platform (as described in the methods section of our manuscript). MALDI-TOF MS data were collected using the Bruker Autoflex Speed TOF Machine. Processed data are uploaded as a compressed file., , # Homeostatic control of deep sleep and molecular correlates of sleep pressure in Drosophila

The zipped file contains 4 folders. The folders are: Figure1, Figures 2 to 4, Figure5 and Figure6.

# Description of the data and file structure

The folder 'Figure1' has three subfolders, i.e., '6h deprivation', '12h deprivation' and '24h deprivation'. Within each of these folders, there are .txt files with the raw sleep data for all individual replicates of the deprived and undisturbed control flies reported in Figure 1 of the manuscript. For instance, Figure1 > 6h deprivation > Control_20sec_SleepData.txt contains the sleep data for undisturbed flies for the 20 second trigger experiment. Similar files for the deprived flies and for different trigger frequencies are uplaoded within each of the three subfolders. The first column in all of these text files is the time information in Zeitgeber Time (ZT) units. Each subsequent column has the individual fly's sleep data.

The folder 'Figures...

睡眠稳态调控通常通过机械刺激诱导强制觉醒，并检测后续睡眠量的增加来实现。但该方法存在一个核心混淆因素：睡眠剥夺引发的生物学反应，可能直接源于机械损伤而非睡眠缺失。各类睡眠剥夺手段均存在此类混淆问题，这也是本领域的重大研究挑战。本文报道了一种适用于果蝇（Drosophila）的新型睡眠剥夺范式，可完全排除睡眠非依赖效应的干扰。研究结果表明，深部睡眠状态是睡眠稳态调控的主要靶标，并证实睡眠剥夺后会出现多周期睡眠反弹现象。此外，本研究证实特异性剥夺深部睡眠状态可引发状态特异性的稳态睡眠反弹。最后，通过考量剥夺实验中机械刺激的分子效应，我们证实血清素水平可追踪睡眠压力（原文此处有截断）。所有行为学数据均通过果蝇活动监测仪（Drosophila Activity Monitors）或Ethoscope平台采集（采集方法详见本文献方法部分）。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）数据通过布鲁克（Bruker）Autoflex Speed飞行时间质谱仪采集。处理后的数据已打包为压缩文件上传。

# 果蝇深部睡眠稳态调控与睡眠压力的分子关联

本压缩包包含4个文件夹，分别为：Figure1、Figures 2至4、Figure5及Figure6。

# 数据与文件结构说明

‘Figure1’文件夹下设3个子文件夹，分别为‘6小时剥夺组’、‘12小时剥夺组’与‘24小时剥夺组’。每个子文件夹内均包含若干.txt格式文件，存储本文献图1中报道的所有剥夺组与未受干扰对照组果蝇的重复样本原始睡眠数据。例如，路径Figure1 > 6h deprivation > Control_20sec_SleepData.txt中存储的是20秒触发实验中未受干扰果蝇的睡眠数据。三个子文件夹内还上传了针对剥夺组果蝇及不同触发频率的同类数据文件。所有文本文件的第一列为授时因子时间（Zeitgeber Time, ZT）单位的时间信息，后续每一列分别对应单只果蝇的睡眠数据。

‘Figures 2至4’文件夹（原文此处未完整给出）