Identification of Eda targets during Meibomian gland development

Eda signaling plays critical role for Meibomian gland dvelopment, however, the crosstalk of Eda with other signaling is largly unknown. By comparing expreession profilings between wild-type and Eda mutant Tabby eyelids, we identified Dkk4 and Lrp6 highly expressed during mouse Meibomian gland development. Total RNAs isolated from microdissected wild-type and Tabby eyelids were profiled with Agilent 44K NIA mouse microarrays.

Eda信号通路（Eda signaling）在睑板腺（Meibomian gland）发育中发挥关键作用，然而目前Eda与其他信号通路的串扰仍未完全明确。通过对比野生型（wild-type）与Eda突变型Tabby小鼠眼睑的表达谱，我们鉴定出在小鼠睑板腺发育过程中高表达的Dkk4与Lrp6。从经显微切割的野生型及Tabby小鼠眼睑中分离得到的总RNA，采用安捷伦（Agilent）44K NIA小鼠微阵列芯片完成了表达谱分析。