Global Transcriptional analysis of human spinal cord and neocortical neuroepithelial stem (NES) cells

We report the transcriptional profile of human spinal cord and neocortical neuroepithelial stem cells (SC-NES and NCX-NES cells) to identify potential engraftment markers in the lesioned spinal cord. We report that cells with a homologous neuroanatomical origin robustly integrate in the host tissue compared to cells with a different regional identity. Specifically, we describe that SC-NES cells display elective integration properties in the lesioned spinal cord compared to NCX-NES cells and that these properties are due to a combination of intrinsic and extrinsic factors. Upon transplantation, SC-NES cells differentiate into neurons and glial cells and extend lond-distance axons, whereas NCX-NES cells remain undifferentiated and display poor integration properties. SC-NES cell upregulate genes related to neurogenesis. In particular. we emphasize the upregulation of MTURN (maturin), ATCAY, PTN (pleiotropin), KAL1 (anosmin), SYT4 and SYT13. We handled 6 samples, 3 for SC-NES cells and 3 for NCX-NES cells. Each sample was handled in triplicate for a total of 18 samples. For SC-NES cells we had: undifferentiated cells in vitro (SC-NES), differentiated for 2 months in vitro (SC-Neurons) and differentiated for two months in vivo upon tranplantation (SC-Graft). Similarly, we had three samples for NCX-NES cells: undifferentiated NCX-NES cells, differentiated in vitro for two months (NCX-Neurons) and differentiated for two months in vivo after transplantation (NCX-Graft)

本研究报道了人脊髓神经上皮干细胞（SC-NES细胞）与新皮质神经上皮干细胞（NCX-NES细胞）的转录组谱，旨在识别损伤脊髓中潜在的移植标记物。研究发现，相较于具有不同区域属性的细胞，具有同源神经解剖起源的细胞可在宿主组织中实现稳定整合。具体而言，相较于NCX-NES细胞，SC-NES细胞在损伤脊髓中展现出特异性整合特性，而该特性由内在与外在因素共同介导。移植后，SC-NES细胞可分化为神经元与胶质细胞，并延伸长距离轴突；而NCX-NES细胞则保持未分化状态，整合能力较差。SC-NES细胞可上调与神经发生相关的基因表达，尤为重要的是，本研究重点报道了MTURN（maturin）、ATCAY、PTN（pleiotropin）、KAL1（anosmin）、SYT4及SYT13的表达上调。本研究共处理6份初始样本，其中SC-NES细胞与NCX-NES细胞各3份；每份样本设置3次生物学重复，总计18份样本。针对SC-NES细胞，样本分为三类：体外未分化状态的SC-NES细胞、体外诱导分化2个月的SC-Neurons细胞，以及移植后体内分化2个月的SC-Graft细胞。同理，NCX-NES细胞的三类样本分别为：未分化NCX-NES细胞、体外诱导分化2个月的NCX-Neurons细胞，以及移植后体内分化2个月的NCX-Graft细胞。