MiSeq 16S rRNA gene (archaeal 16S rRNA gene) sequences of 33 Mai Po sediments, including 18 samples from winter and 15 samples from summer

NIAID Data Ecosystem2026-05-01 收录

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https://www.ncbi.nlm.nih.gov/sra/ERP013906

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DNA from 33 Mai Po sediments have been extracted and amplified by 21f/958r & Arch347F/Arch806R primer pairs (for archaeal 16S rRNA gene). The archaeal communities of these 33 samples were obtained. All samples were sequenced in a pair-end layout (PE 250bp or 300bp by MiSeq). Before submission, we have merged them into one read using FLASH-1.2.8. After merging, one individual read is about ~420bp. Sample ID to read file map: Sample ID Read file MG1SumA ZZCAS1 MG1SumB ZZCAS2 MG1SumC ZZCAS3 MG2SumA ZZCAS4 MG2SumB ZZCAS5 MG2SumC ZZCAS6 MG3SumA ZZCAS7 MG3SumB ZZCAS8 MG3SumC ZZCAS9 TF1SumA ZZCAS10 TF1SumB ZZCAS11 TF2SumA ZZCAS12 TF2SumB ZZCAS13 TF3SumA ZZCAS14 TF3SumB ZZCAS15 MG1WinA ZZCAW1 MG1WinB ZZCAW2 MG1WinC ZZCAW3 MG1WinD ZZCAW4 MG2WinA ZZCAW5 MG2WinB ZZCAW6 MG2WinC ZZCAW7 MG2WinD ZZCAW8 MG3WinA ZZCAW9 MG3WinB ZZCAW10 MG3WinC ZZCAW11 MG3WinD ZZCAW12 TF1WinA ZZCAW13 TF1WinB ZZCAW14 TF2WinA ZZCAW15 TF2WinB ZZCAW16 TF3WinA ZZCAW17 TF3WinB ZZCAW18

本数据集从33份米埔（Mai Po）湿地沉积物样本中提取基因组DNA，并通过21f/958r与Arch347F/Arch806R引物对（primer pairs）完成扩增，该引物对靶向古菌16S核糖体RNA基因（archaeal 16S rRNA gene）。本研究成功获取了这33份样本的古菌群落组成信息。所有样本均采用MiSeq测序平台进行双端测序模式（Pair-end layout, PE），测序读长为250 bp或300 bp。在序列提交前，本研究使用FLASH-1.2.8软件将双端测序读段拼接为单条合并序列，拼接后单条序列长度约为420 bp。样本ID与测序读段文件的对应关系如下：样本ID 读段文件 MG1SumA ZZCAS1 MG1SumB ZZCAS2 MG1SumC ZZCAS3 MG2SumA ZZCAS4 MG2SumB ZZCAS5 MG2SumC ZZCAS6 MG3SumA ZZCAS7 MG3SumB ZZCAS8 MG3SumC ZZCAS9 TF1SumA ZZCAS10 TF1SumB ZZCAS11 TF2SumA ZZCAS12 TF2SumB ZZCAS13 TF3SumA ZZCAS14 TF3SumB ZZCAS15 MG1WinA ZZCAW1 MG1WinB ZZCAW2 MG1WinC ZZCAW3 MG1WinD ZZCAW4 MG2WinA ZZCAW5 MG2WinB ZZCAW6 MG2WinC ZZCAW7 MG2WinD ZZCAW8 MG3WinA ZZCAW9 MG3WinB ZZCAW10 MG3WinC ZZCAW11 MG3WinD ZZCAW12 TF1WinA ZZCAW13 TF1WinB ZZCAW14 TF2WinA ZZCAW15 TF2WinB ZZCAW16 TF3WinA ZZCAW17 TF3WinB ZZCAW18

创建时间：

2023-04-26