rds file and code for scRNA-seq analysis

This program aims to investigate the role of CD4+ T cell in chronic colitis model. Total immune cells were sorted from the colonic pathces of Rag1-/- mice which received naive CD4+ T cells after 4 weeks for Single-cell RNA-seq processing.

本研究旨在探究CD4+ T细胞（CD4+ T cell）在慢性结肠炎模型中的作用。将初始CD4+ T细胞（naive CD4+ T cells）输注至Rag1基因敲除小鼠（Rag1-/- mice）体内后，于造模后4周，从该类小鼠的结肠淋巴集结中分选得到总免疫细胞，用于单细胞RNA测序（Single-cell RNA-seq）实验。