Impact of CDK7 inhibition across a range of inhibitors in the context of heat shock

Here we evaluate the effect of CDK7 inhibition in the context of heat shock across three distinct inhibitors. Two inhibitors are Syros compounds SY-5609 and SY-5102. The final inhibitor is 3MBPP1 in a CDK7 analog sensitive cell line. We find that overall the same gene programs are impacted. Overall design: RNA-seq in OV90 cells to evaluate the effects of CDK7 inhibition under HS stimulated conditions. There are 3 biological replicates for each condition. Inhibitors here include SY-5102 and 3MBPP1 (in an OV90 CDK7-analog sensitive cell line).

本研究针对热应激（heat shock）情境下的三种不同抑制剂，评估了细胞周期蛋白依赖性激酶7（CDK7）的抑制效应。其中两款抑制剂为Syros公司的SY-5609与SY-5102化合物，第三款抑制剂为3MBPP1，作用于CDK7类似物敏感细胞系。研究发现，整体而言受影响的基因程序保持一致。实验整体设计：在OV90细胞中开展RNA测序（RNA-seq），以评估热应激（HS）刺激条件下CDK7抑制的效应。每组设置3次生物学重复。本次实验所用抑制剂包括SY-5102以及作用于OV90 CDK7类似物敏感细胞系的3MBPP1。