Be1 and Be2 B cells are transcriptionally distinct

B cells cultured in the presence of Th1 CD4 T cells (Be1) or Th2 CD4 T cells (Be2) lead to distinct phenotypic outcomes. To determine when the phenotypic differences emerged, IgD positive and IgD negative Be1 and Be2 cells were isolated from cultures derived from 3 independent donors and RNA-seq performed. These data define the transcriptional differences of Be1 and Be2 cells ad distinct differentiation stages and show that Be1 B cells contain a pre-ASC signature that is absent from Be2 B cells. Be1 and Be2 cells that were IgD positive and IgD negative from three different donors were analyzed by RNAseq.

在1型辅助性CD4阳性T细胞（Th1 CD4 T cells）或2型辅助性CD4 T细胞（Th2 CD4 T cells）共培养体系中培育的B细胞（B cells），可呈现出截然不同的表型特征。为明确该表型差异的出现时机，研究人员从3名独立供体来源的共培养体系中分离出IgD阳性与IgD阴性的Be1、Be2细胞，并开展了RNA测序（RNA-seq）。本数据集明确了Be1与Be2细胞在不同分化阶段的转录组差异，并证实Be1 B细胞携带有Be2 B细胞所不具备的pre-ASC特征（pre-ASC signature）。研究人员对3名不同供体来源的IgD阳性与IgD阴性Be1、Be2细胞开展了RNA测序（RNA-seq）分析。