differential expression analysis in dermal fibroblasts of twins discordant for scleroderma. differential expression analysis in dermal fibroblasts of twins discordant for scleroderma

comparison of the transcriptomic signature in healthy and SSc affected twins, including by disease subtype (limited or diffuse) Overall design: dermal fibroblasts were outgrown from skin biopsies of healthy and scleroderma affected twins, total RNA was isolated for RNAseq analysis,

健康个体与系统性硬化症（SSc）受累双胞胎的转录组特征比较，涵盖疾病亚型（局限性或弥漫性）。整体实验设计：从健康及系统性硬化症受累双胞胎的皮肤活检样本中体外扩增培养真皮成纤维细胞，提取总RNA用于RNA测序（RNAseq）分析。