Bifidobacterium longum promotes postoperative liver function recovery in patients with hepatocellular carcinoma

This study is constructed by three cohorts which approved by the Ethics Committee of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (Wuhan China). First, a retrospective cohort study (490 patients) was designed and implemented to investigate the prognostic impact of postoperative liver function recovery. Second, a prospective observational cohort study (123 patients, ClinicalTrials number: NCT04303286) was conducted to identify key gut microbial species associated with postoperative liver function recovery. Finally, an open, randomized, blank controlled clinical trial (176 patients, ClinicalTrials.gov identifier: NCT05178524) was performed to test whether a key gut microbial species that was enriched in the normal recovery group compared with the delayed recovery group (i.e., Bifidobacterium longum) can promote the postoperative liver function recovery in HCC patients. At the same time, the effectiveness and mechanism of B. longum on postoperative liver function recovery were verified by using a liver- hepatectomy mouse model. These databases including original western blot images and metabolic data (related to Figures 6 and 7).

本研究包含三项经华中科技大学同济医学院附属同济医院伦理委员会（中国武汉）批准的队列研究。第一项为回顾性队列研究，纳入490例患者，旨在探究术后肝功能恢复对患者预后的影响。第二项为前瞻性观察队列研究，纳入123例患者，临床试验注册号为NCT04303286，旨在筛选与术后肝功能恢复相关的关键肠道菌群物种。第三项为开放标签、随机、空白对照临床试验，纳入176例患者，临床试验注册号为NCT05178524，旨在验证相较于延迟恢复组，正常恢复组中富集的关键肠道菌群物种（即长双歧杆菌(Bifidobacterium longum)）是否可促进肝细胞肝癌（HCC）患者术后肝功能恢复。同时，本研究通过肝切除术小鼠模型，验证了长双歧杆菌（B. longum）促进术后肝功能恢复的有效性及作用机制。本研究数据集包含原始蛋白免疫印迹（Western Blot）图像及与图6、图7相关的代谢数据。