Epstein-Barr Virus Nuclear Antigen 1 (EBNA1). Homo sapiens

Expression of mRNAs in EBV-positive B-cell strains in the presence or absence of EBNA1 or a mutant of EBNA1 (ΔUR1-EBNA) that is unable to activate transcription. Overall design: B-cell strains derived from 2 donors (numbers 20 and 27) infected with WT-EBV and ΔEBNA1-EBV and an unrelated third donor infected with EBV carrying ΔUR1-EBNA1. Each done in biological triplicate. Samples were normalized to the expression of mRNAs in a pool of RNAs from B-cells from donors 20 and 27 infected with ΔEBNA1-EBV

本数据集针对爱泼斯坦-巴尔病毒（Epstein-Barr Virus, EBV）阳性B细胞株，在存在或缺失EB病毒核抗原1（Epstein-Barr Nuclear Antigen 1, EBNA1），或携带无法激活转录的EBNA1突变体（ΔUR1-EBNA）时的信使RNA（messenger RNA, mRNA）表达谱展开分析。整体实验设计如下：实验所用B细胞株来源于2名编号为20和27的供体，分别感染野生型EB病毒（Wild-type EBV, WT-EBV）与缺失EBNA1的EB病毒（ΔEBNA1-EBV）；另有1名无关供体来源的B细胞株，感染携带ΔUR1-EBNA1的EB病毒。所有实验组均设置三次生物学重复。所有样本均以编号20与27的供体感染ΔEBNA1-EBV的B细胞总RNA混合池的mRNA表达水平作为参照完成标准化处理。