Multi-colour SMLM images of Escherichia coli cells with varying RNaseE localization and expression (LB, exponential phase)

This dataset is part of the publication "The nucleoid of rapidly growing Escherichia coli localizes close to the inner membrane and is organized by transcription, translation and cell geometry".

It contains the SMLM images of RNase E experiments, in which the localization and abundance of RNase E was altered. It also includes the regions of interest used for the analyses.

Cultures were then chemically fixed, permeabilised and imaged for the nucleoid (JF646-Hoechst) and membranes (Nile Red) using PAINT. Strains MBS106 (NCM3416, rne-frt) (Reference), MBS157 (NCM3416, rneΔMTS-frt) (Reference) and SLP60 (pKti4/Δrne-frt) were kind gifts of Agamemnon J. Carpousis.

More information can be found in the publication.

本数据集隶属于学术论文《快速生长大肠杆菌的类核定位于内膜附近，并由转录、翻译及细胞几何结构调控组装》。
数据集包含核糖核酸酶E（RNase E）实验的单分子定位显微镜（SMLM）图像，该实验中核糖核酸酶E的定位与丰度均发生改变。此外还包含分析所用的感兴趣区域。
随后对培养物进行化学固定、透化处理，并采用PAINT成像技术对类核（JF646-赫斯特（Hoechst）染料）与细胞膜（尼罗红染料）进行标记成像。所用菌株MBS106（NCM3416, rne-frt）（参考菌株）、MBS157（NCM3416, rneΔMTS-frt）（参考菌株）及SLP60（pKti4/Δrne-frt）均由Agamemnon J. Carpousis惠赠。
更多详细信息可参阅该学术论文。