scATAC-seq of Inguinal Adipose Tissue of UCP1-KO and WT mice.

Single cell level analysis of transcriptional circuits regulating UCP1-indepednent theremogensis through Lgr6 in inguinal Adipose tissue. We previously identified an increase in Lgr6 gene expression in the beige adipocyte population of UCP1-KO mice and hypothesized its involvement in UCP1-independent thermogensis. Consistent with our previous data the scATAC-seq data reveals that chromatin accessibility at the Lgr6 loci is significantly higher in Ucp1- KO beige adipocytes as compared to WT and the promter is also more accessible in Ucp1- KO beige adipocytes. Other DNA-binding motifs involved in UCP-1 indepedent thermogensis were also analyzed and found to be upregulated in the KO model. Comaparison of UCP1-KO and WT mice under cold exposure.

本研究针对腹股沟脂肪组织中通过Lgr6调控不依赖解偶联蛋白1（UCP1）的产热的转录环路开展单细胞水平分析。我们此前已在UCP1敲除（UCP1-KO）小鼠的米色脂肪细胞（beige adipocyte）群中检测到Lgr6基因表达上调，并推测其参与不依赖UCP1的产热过程。本研究的单细胞转座酶可及性测序（scATAC-seq）数据与此前的研究结果一致：相较于野生型（Wild Type，WT）小鼠，UCP1敲除米色脂肪细胞中Lgr6基因位点的染色质可及性显著升高，其启动子区域的染色质可及性同样更高。此外，本研究还对参与不依赖UCP1产热过程的其他DNA结合基序进行了分析，结果显示其在敲除模型中表达上调。本研究还开展了寒冷暴露条件下UCP1敲除与野生型小鼠的对照分析。