Transcriptome analyses reveal epigenetic and radiation related genes in NSCLC cell. Transcriptome analyses reveal epigenetic and radiation related genes in NSCLC cell

Purpose: Radiotherapy is useful for non-small cell lung cancer (NSCLC) patients who cannot be treated surgically. Modification of histone proteins occurs during radiotherapy, and affects gene expression.In this study, we assessed the effects of radiotherapy on histone modification via 3-deazaneplanocin A (DZNep) in NSCLC cells. Methods: NCI-H460 NSCLC cell line were subjected to gamma irradiation. To reveal histone modification reagent DZNep targeted genes, we conducted mRNA-sequencing. Results: We evaluated the epigenetic regulation of autophagy-related genes by DZNep through mRNA-sequencing, and we identified genes that are differentially expressed upon irradiation, including a candidate histone modification target gene. Overall design: We subjected DZNep- or DMSO-treated NCI-H460 cells exposed to 0 or 4 Gy of radiation to mRNA-seq and searched for differently differentially expressed genes.研究目的：放射治疗可用于无法接受手术治疗的非小细胞肺癌（non-small cell lung cancer, NSCLC）患者。放疗过程中会发生组蛋白修饰改变，并对基因表达产生调控作用。本研究借助3-脱氮腺苷（3-deazaneplanocin A, DZNep），探究放疗对非小细胞肺癌细胞内组蛋白修饰的影响。 方法：将NCI-H460非小细胞肺癌细胞系进行γ射线照射处理。为明确组蛋白修饰试剂DZNep的靶基因，本研究开展了mRNA测序（mRNA-sequencing）实验。 结果：通过mRNA测序分析DZNep对自噬相关基因的表观遗传调控作用，并鉴定出辐射处理后差异表达的基因，其中包含一个候选组蛋白修饰靶基因。 整体实验设计：我们对经DZNep或二甲基亚砜（dimethyl sulfoxide, DMSO）处理、并接受0 Gy或4 Gy辐射的NCI-H460细胞开展mRNA测序，以筛选差异表达基因。