Cold atmospheric plasma in sensitizing therapy towards breast cancer

This collection comprises a comprehensive dataset presenting the chemical and biological activity of cold atmospheric plasma (CAP) systems generated in a helium atmosphere. The study was conducted using two plasma configurations: (i) a surface atmospheric pressure plasma jet (surface APPJ, denoted as SCAP) and (ii) a custom-designed, 3D-printed module enabling subcutaneous plasma delivery (denoted as NCAP).The dataset focuses on evaluating the oxidative potential of the plasma systems, quantifying the generation of key reactive oxygen and nitrogen species (RONS), and assessing their biological effects on human cell lines, including non-tumorigenic MCF10A cells and breast cancer cell lines MCF7 and MDA-MB-231.The research was carried out במסגרת the Miniatura 8 project (02NA/0020/24), funded by the National Science Centre (Poland). The overarching objective of this project is to determine the potential of cold atmospheric plasma technology to sensitize breast cancer cells to selected anticancer agents, including L-ascorbic acid, curcumin, and salinomycin.Contents of the DatasetThe collection includes the following experimental data:UV–Vis spectrophotometric measurements of organic compound degradation, including methylene blue and 4-nitrophenol, used as model systems to evaluate the oxidative capacity of CAP.UV–Vis absorbance measurements of chromogenic products formed via selective reactions with key RONS species, including hydrogen peroxide (H₂O₂), nitrite (NO₂⁻), and total ROS.Total ROS quantification includes scavenger-based masking experiments using D-mannitol, tiron monohydrate, and sodium pyruvate.Optical Emission Spectroscopy (OES) spectra characterizing the gas-phase composition of the plasma discharge.Sulforhodamine B (SRB) assay results evaluating the viability of MCF10A, MCF7, and MDA-MB-231 cells following exposure to CAP and selected anticancer agents.Scratch (wound healing) assay data assessing cell migration and motility under CAP treatment and combination therapies.Flow cytometry measurements (Annexin V/Propidium Iodide staining) for the evaluation of apoptotic and necrotic cell populations, as well as autophagy-related processes.Intracellular RONS measurements, providing quantitative data on oxidative and nitrosative stress induced by plasma exposure.Supplementary graphical materials, including:3D design files and visualizations of the subcutaneous plasma delivery module,photographic documentation of biological experiments and cellular responses.

本数据集收录了一套完整的实验数据，呈现了氦气氛围中产生的冷大气等离子体（cold atmospheric plasma, CAP）系统的化学与生物活性。本研究采用两种等离子体配置开展实验：(i) 表面型大气压等离子体射流（surface atmospheric pressure plasma jet, surface APPJ，记为SCAP）；(ii) 定制设计的3D打印皮下等离子体输送模块（记为NCAP）。本数据集围绕等离子体系统的氧化潜能评估、关键活性氧氮物种（reactive oxygen and nitrogen species, RONS）生成量定量，以及其对人类细胞系的生物学效应评价展开，所涉细胞系包括非致瘤性MCF10A细胞与乳腺癌细胞系MCF7、MDA-MB-231。本研究依托波兰国家科学中心资助的Miniatura 8项目（编号：02NA/0020/24）开展，该项目的总体目标是明确冷大气等离子体技术使乳腺癌细胞对选定抗癌药物（包括L-抗坏血酸、姜黄素与沙利霉素）产生致敏性的潜力。

数据集内容
本数据集包含如下实验数据：
以亚甲基蓝与4-硝基苯酚为模型体系，开展有机化合物降解的紫外-可见分光光度检测，用于评估CAP的氧化能力；
针对关键RONS物种（包括过氧化氢（H₂O₂）、亚硝酸盐（NO₂⁻）与总活性氧（ROS））的选择性反应所生成的显色产物，开展紫外-可见吸光度检测；
总ROS定量分析数据涵盖基于清除剂的掩蔽实验结果，所用清除剂包括D-甘露醇、钛铁试剂单水合物与丙酮酸钠；
表征等离子体放电气相组分的发射光谱（Optical Emission Spectroscopy, OES）数据；
磺酰罗丹明B（Sulforhodamine B, SRB）实验结果，用于评估MCF10A、MCF7与MDA-MB-231细胞在暴露于CAP及选定抗癌药物后的细胞活力；
划痕（伤口愈合）实验数据，用于评估CAP处理及联合疗法下的细胞迁移与运动能力；
流式细胞术检测数据（采用膜联蛋白V/碘化丙啶染色法），用于评估细胞凋亡与坏死群体，以及自噬相关过程；
细胞内RONS检测数据，可提供等离子体暴露诱导的氧化应激与亚硝化应激的定量信息；
补充可视化材料，包括：
皮下等离子体输送模块的3D设计文件与可视化效果图；
生物学实验与细胞响应的影像记录资料。