Defining the developmental program leading to meiosis in maize. Defining the developmental program leading to meiosis in maize

We isolated pre-meiotic and early meiotic cells from 24 maize anthers, covering a week of development from the day after archesporial (AR) cell specification to the early zygotene stage of meiotic prophase I. Starting material was staged by anther length, and anther stages were densely sampled from throughout this period. High quality reads were obtained from 144 cells. Overall design: Single-cell RNA-seq of premeiotic and early meiotic cells from maize anthers. Libraries were constructed with a modified version of CelSeq2.

本研究从24份玉米花药中分离得到减数分裂前细胞与减数分裂早期细胞，样本覆盖了从孢原细胞（archesporial, AR）特化次日至减数分裂I前期早期偶线期的整一周发育进程。研究通过花药长度对起始实验材料进行发育分期，并在该发育周期内对花药各阶段开展了密集采样。最终从144个细胞中获得了高质量测序读段。研究总体设计如下：针对玉米花药的减数分裂前及早期减数分裂细胞实施单细胞RNA测序；文库构建采用了改良版CelSeq2。