Presence of human serum in vitro enhances NTS viability and induces worm development past the LuS.

NTS were cultured in HM and DMEM supplemented with 200 U/ml Penicillin and 200 μg/ml Streptomycin with or without additional supplementation of 20% HSe. (A) Viability scoring was performed at the indicated time points. The percentage of developmental stages in culture with either (B) HM, (C) HM + 20% HSe, (D) DMEM or (E) DMEM + 20% HSe was calculated for the indicated time points by bright field microscopy. (F) Representative photomicrographs were taken on day 35 post-transformation. Scale bar applies to all shown pictures. Arrowheads indicate dead NTS. Arrows indicate early and late LiS. Each data point is shown as a mean ± SD of pooled data from three independent experiments with three biological replicates each. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 comparing HM with HM + 20% HSe, × p ≤ 0.05, ×× ≤ 0.01, ××× ≤ 0.001 comparing DMEM with DMEM + 20%. HSe, human serum; SkS, skin stage; LuS, lung stage; LiS, liver stage; s.p., score points; p.t., post-transformation. (PZFX)将NTS置于添加了200 U/ml青霉素与200 μg/ml链霉素的HM培养基及DMEM培养基中培养，同时分别添加或不添加20% HSe。(A) 在指定时间点开展活力评分。针对以下四种培养体系：(B) HM培养基、(C) HM + 20% HSe培养基、(D) DMEM培养基、(E) DMEM + 20% HSe培养基，通过明场显微镜在指定时间点计算各发育阶段的占比。(F) 于转化后第35天拍摄代表性显微照片，所有展示图片共用该标尺。箭头头标注死亡的NTS，箭头标注早期与晚期LiS（liver stage）。所有数据点以三次独立实验的合并数据的均值±标准差呈现，每次实验包含三个生物学重复。对比HM与HM + 20% HSe组时，* p ≤ 0.05，** p ≤ 0.01，*** p ≤ 0.001；对比DMEM与DMEM + 20% HSe组时，× p ≤ 0.05，×× p ≤ 0.01，××× p ≤ 0.001。缩写说明：HSe为人血清（human serum）；SkS为皮肤期（skin stage）；LuS为肺期（lung stage）；LiS为肝期（liver stage）；s.p.为评分点；p.t.为转化后（post-transformation）。(PZFX)