BaRTv2: A highly resolved barley reference transcriptome for accurate transcript-specific RNA-seq quantification

BaRTv2 represents a new and improved Reference Transcript Dataset built upon a broad framework of highly informative Pacbio Iso-seq full length transcripts incorporating accurate SJ, TSS and TES information. It is based upon the European 2-row spring barley cv. Barke which itself is highly relevant to contemporary barley agriculture and current research. The combination of the novel Iso-seq analysis and filtering pipeline, the new automated quality control pipeline for short read assembled transcripts and detailed transcript characterisation and annotation make BaRTv2 a high-quality community-wide reference dataset.

BaRTv2是一款全新改良的参考转录本数据集（Reference Transcript Dataset），其构建依托于一套由高信息量Pacbio Iso-seq全长转录本构成的广泛框架，该框架整合了精准剪接位点（Splice Junction, SJ）、转录起始位点（Transcription Start Site, TSS）与转录终止位点（Transcription End Site, TES）信息。该数据集以欧洲二棱春大麦cv. Barke为研究材料，而该品种本身与当代大麦农业及当前科研方向高度相关。结合全新的Iso-seq分析与过滤流程、针对短读长组装转录本的新型自动化质控流程，以及详尽的转录本表征与注释工作，BaRTv2已成为一套高质量的面向全科研社区的参考数据集。