The salivary microbial communities in periodontal health and periodontitis studied by sequencing the V3-V4 16S rRNA gene region.

The present bioproject contains 58 saliva samples obtained from subjects with different periodontal health conditions, which were analysed by amplifying and sequencing the V3-V4 16S rRNA gene region using an Illumina MiSeq instrument.The experiment consisted of 22 periodontally healthy and 36 periodontitis patients. Among the latter, 2 were classified as moderate and 34 as severe. The periodontitis cases were diagnosed according to the classification of Page and Eke (J Periodontol. 2007; 78:1387-99). The attached metadata table contains information regarding the following variables for each participant: health condition, periodontitis type (if applicable), periodontitis severity (if applicable), sample type, saliva type, paired design, therapy, sample time after the diagnosis, age, sex, ethnicity, systemic condition, smoking habit (including number of cigarettes/day and months smoking), and clinical parameters (total number of teeth, plaque index, bleeding on probing, probing pocket depth and clinical attachment level).The study subjects were aged between 30 and 79 years old, 34 of them were males and 24 females, and there were 28 non-smokers and 30 smokers. All individuals were systemically healthy and Caucasian.Also 3 mock samples named HM276, HM277, and D6303, were included to be used as quality control of sequences.

本生物项目包含58份取自不同牙周健康状态受试者的唾液样本，采用Illumina MiSeq仪器对V3-V4区16S rRNA基因片段进行扩增并测序以完成样本分析。本实验共纳入22名牙周健康受试者与36名牙周炎患者，其中2例为中度牙周炎、34例为重度牙周炎。牙周炎病例的诊断依据Page与Eke于2007年发表于《牙周病学杂志》的分类标准（J Periodontol. 2007; 78:1387-99）。所附元数据（metadata）表包含每名受试者的如下变量信息：健康状态、牙周炎类型（若适用）、牙周炎严重程度（若适用）、样本类型、唾液类型、配对设计信息、治疗方案、诊断后采样时间、年龄、性别、种族、全身健康状况、吸烟习惯（含每日吸烟支数与吸烟时长），以及临床参数：总牙数、菌斑指数、探诊出血情况、探诊深度与临床附着水平。本研究受试者年龄介于30至79岁之间，其中男性34名、女性24名，非吸烟者28名、吸烟者30名；所有受试者均无系统性基础疾病，且为高加索人种。此外还纳入3份分别命名为HM276、HM277与D6303的模拟样本，用于作为测序序列的质量对照。