Gene expression analysis in T cells from spleens of mice that provide a model for systemic lupus erythematosus. Mus musculus

This laboratory focuses on the interactions of murine CD4+CD25+ regulatory T cells (Tregs) with CD4+CD25- T cells, both from a fundamental biological point of view, and in the context of a murine model of lupus. Overall design: This study characterized the surface glycans expressed by CD25 and CD25- CD4 T cells selected from lupus-prone (systemic lupus erythematosus or SLE) mouse strains MRL/Mp and NZB/W F1 and two control strains, CBA/Ca (haplotype-matched with the MRL/Mp; H-2k) and BALB/c. A transcriptomic survey of freshly isolated and bead-activated CD25 and CD25- CD4 T cells selected from mouse spleens was conducted using these four strains. Three replicate samples of each condition were hybridized to the GLYCOv2 array, for a total of 48 samples.

本实验室聚焦于小鼠CD4+CD25+调节性T细胞（regulatory T cells, Tregs）与CD4+CD25- T细胞的相互作用，研究维度既涵盖基础生物学层面，也包含狼疮小鼠模型相关的应用探索。
实验整体设计：本研究首先对狼疮易感（系统性红斑狼疮，systemic lupus erythematosus, SLE）小鼠品系MRL/Mp、NZB/W F1，以及两个对照品系——与MRL/Mp单倍型匹配的CBA/Ca（H-2k）和BALB/c——中筛选得到的CD25+与CD25- CD4 T细胞所表达的表面聚糖进行了表征；随后，本研究使用上述四个品系的小鼠，对从其脾脏中分离的新鲜及磁珠活化的CD25+与CD25- CD4 T细胞开展了转录组学分析。每个实验条件均设置3个生物学重复样本，将其与GLYCOv2芯片进行杂交，总计完成48个样本的杂交实验。