Ca2+ release-activated Ca2+ entry is a potent host innate immune defence against influenza A virus. Ca2+ release-activated Ca2+ entry is a potent host innate immune defence against influenza A virus

Gene expression was determined for normal human bronchial epithelial (NHBE) cells incubated with DMSO or TG immediately prior to infection with USSR H1N1 virus for 12 hours. All of the NHBE cell stocks were obtained from QIAGEN. Overall design: Normal human bronchial epthelial cells mRNA profiles were generated by deep sequencing, in quadruplicate (two donors and two replicates per donor), using Illumina HiSeq 3000

本研究针对经二甲基亚砜（Dimethyl Sulfoxide, DMSO）或TG预处理后、即刻用苏联H1N1病毒感染并持续培养12小时的正常人支气管上皮细胞（Normal Human Bronchial Epithelial, NHBE），完成了基因表达水平检测。所有NHBE细胞株均购自凯杰（QIAGEN）公司。整体实验设计：采用Illumina HiSeq 3000测序平台开展深度测序，以获取正常人支气管上皮细胞的mRNA表达谱；实验设置四重生物学重复，包含2名供体来源的细胞，且每名供体的细胞设置2次重复。