CROP-Seq in Primary Human T Cells

We adapted sgRNA lentiviral infection and Cas9 electroporation (SLICE) to allow for CROP-Seq (Datlinger et al, Nat Med 2017) in primary cells. We used a library of 48 sgRNA, derived from GW screens, to explore transcriptional changes downstream of CRISPR-KO. Pooled CRISPR screen with single cell RNASeq readout (Perturb-Seq / CROP-Seq) in primary human T cells. Dataset includes CD8 T cells from two donors, for two conditions: with TCR stimulation or No stimulation.我们对单导RNA（sgRNA）慢病毒感染与Cas9电穿孔（SLICE）技术进行适配改造，使其可在原代细胞中开展CROP-Seq实验（Datlinger等，《自然·医学》，2017）。我们采用一套源自全基因组筛选（GW screens）的48条sgRNA文库，以探究CRISPR基因敲除（CRISPR-KO）下游的转录组变化。本数据集针对原代人T细胞开展了基于单细胞RNA测序读长的混合CRISPR筛选（Perturb-Seq / CROP-Seq）。数据集包含来自2名供体的CD8阳性T细胞，涵盖2种培养条件：T细胞受体（TCR）刺激组与未刺激组。