Analysis of plant derived alkaloids on rumen microbiota using an in vitro Rusitec system

Rumen microbiota have important metabolic functions for the host animal. This study aimed at characterizing changes in rumen microbial abundances and predicted metabolic pathways using an in vitro model, and to evaluate a potential modulatory role of plant derived alkaloids (PDA) using MiSeq paired ends sequencing and PICRUSt predicitve metabolic pathway analysis. The measurement of community diversity showed an exponential increase and eventual plateau for all samples. Beta diversity analysis showed high levels of variation between individual fermenters, resulting in high variation within treatments.

瘤胃微生物群（rumen microbiota）对宿主动物具有重要的代谢功能。本研究旨在借助体外模型表征瘤胃微生物丰度与预测代谢通路的变化，并通过MiSeq双端测序及PICRUSt预测性代谢通路分析，评估植物源生物碱（plant derived alkaloids, PDA）的潜在调控作用。所有样本的群落多样性检测结果均呈现指数级增长，最终趋于平稳。β多样性（Beta diversity）分析显示，单个发酵罐间存在较高的异质性，进而导致处理组内的差异较大。