Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids

Abstract: The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes.摘要：本研究旨在通过基因组原位杂交（genomic in situ hybridization, GISH）技术，评估两种经人工多倍体化的象草×珍珠粟种间杂种的染色体配对构型，以此作为不同亲本基因组间染色体发生重组的潜在指示指标。实验以幼小花蕾的花药作为染色体制备材料。基因组探针以珍珠粟DNA制备，并通过切口平移反应标记上生物素-16-脱氧尿苷三磷酸（biotin-16-dUTP）。阻断DNA采用象草基因组DNA制备。在两种杂种中观察到的部分同源基因组间配对现象，表明亲本基因组的染色体之间存在发生重组的可能性。