Transcriptome analysis of iron and phosphorus interaction in rice seedlings. Oryza sativa

The antagonistic interaction between iron (Fe) and phosphorus (P) has been noted in the area of plant nutrition. To understand the physiology and molecular mechanisms of this interaction, we studied the growth performance, nutrient concentration, and gene expression profiles of root and shoot segments derived from 10-d-old rice (Oryza sativa) seedlings under four different nutrient conditions: (1) full strength of Fe and P (+Fe+P); (2) full strength of P and no Fe (-Fe+P); (3) full strength of Fe and no P (+Fe-P); and (4) without both Fe and P (-Fe-P). We used Affymetrix genechip to detail the global programme of gene expression underlying different Fe and P supply conditions identified distinct classes of up-regulated genes. Overall design: The GeneChip analysis experiment was designed as a two-factor experiment with four treatments: (1) +P+Fe; (2) –Fe+P; (3) +Fe–P; (4) –Fe–P. The experiment included two biological replicates. Each sample represented root and shoot material from five to 10 plants.

铁（Fe）与磷（P）之间的拮抗交互作用在植物营养学领域已有研究报道。为阐明该交互作用的生理与分子机制，本研究以生长10天的水稻（Oryza sativa）幼苗的根、茎切段为实验材料，设置四种不同养分培养条件：(1) 全量铁与全量磷（+Fe+P）；(2) 全量磷且无铁（-Fe+P）；(3) 全量铁且无磷（+Fe-P）；(4) 无铁且无磷（-Fe-P），随后分析其生长表现、养分浓度及基因表达谱。本研究采用Affymetrix基因芯片（Affymetrix GeneChip）详细解析不同铁、磷供应条件下的全局基因表达模式，鉴定得到多个类别的上调基因。总体实验设计：本次基因芯片分析实验为双因素实验，包含上述四种处理，设置2个生物学重复。每份样本取自5~10株幼苗的根与茎组织。