Flow cytometry of FISH samples

Higer order taxonomic groups were labelled by Fluorescent in situ hybridisation (FISH) in mixed microbial communities to evaluate the community composition over time. The FISH labelling increases the information in the fingerprint by adding taxonomic and translational information next to the phenotypic information.
Conclusion:
FISH adds information to our flow cytometric fingerprint that enables more thourough understanding of the community dynamics

研究人员借助荧光原位杂交（Fluorescent in situ hybridisation，FISH）对混合微生物群落中的高阶分类群（higher order taxonomic groups）进行标记，以此评估群落组成随时间的动态变化。FISH标记通过在表型信息之外额外添加分类学信息与翻译组信息，提升了流式细胞术指纹图谱的信息量。
结论：
FISH为我们的流式细胞术指纹图谱增添了有效信息，可助力更为全面透彻地解析群落动态变化。