RNA-Sequencing of ST2+ Treg cells in response to IL-33 stimulation

The effects of IL-33 on ST2+ Treg cells were not studied thouroughly. We FACS-sorted in vitro expanded ST2+ Treg cells from C57BL/6 Foxp3-IRES-mRFP (B6 FIR) mice. We next used RNA-seq techonology to define how recombinant IL-33 (rIL-33) may impact mouse Treg by to assessing the transcriptome of IL-33-stimulated ST2+ Treg cells compared to that of untreated ST2+ Treg cells. Our data revealed that ST2+ Treg stimulated with rIL-33 for 6 hours exhibited increased expression of Il10 and Il13 compared to unstimulated ST2+ Treg cells. Examine of transcriptome of rIL-33-treated or untreated ST2+ Treg cells.

白介素33（IL-33）对ST2+ Treg细胞（ST2+ Treg cells）的作用尚未得到深入研究。本研究从C57BL/6 Foxp3-IRES-mRFP（B6 FIR）小鼠中分离获得经体外扩增的ST2+ Treg细胞，并通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）完成纯化。随后，本研究采用RNA测序（RNA-seq）技术，通过对比经重组IL-33（rIL-33）刺激的ST2+ Treg细胞与未处理ST2+ Treg细胞的转录组，解析rIL-33对小鼠Treg细胞的调控效应。研究数据显示，与未受刺激的ST2+ Treg细胞相比，经rIL-33刺激6小时的ST2+ Treg细胞中，Il10与Il13的表达水平显著上调。本研究针对经rIL-33处理或未处理的ST2+ Treg细胞的转录组展开了分析。