De novo nanopore sequencing overrepresents RNA modification landscape, part 3

RNA modifications are critical to the functional diversity and regulatory complexity of the transcriptome. With increasing frequency, direct nanopore RNA sequencing is applied to identify RNA modifications de novo. Here, we directly compare the MS2 phage genome RNA modification profiles determined using nanopore to orthogonal LC-MS/MS assays. The results reveal very different views of the modification landscape, suggesting caution when calling new RNA modifications using nanopore alone.RNA修饰（RNA modifications）对于转录组的功能多样性与调控复杂性至关重要。当前，直接纳米孔RNA测序（nanopore RNA sequencing）正被愈发频繁地应用于从头（de novo）识别RNA修饰。本研究直接对比了通过纳米孔测序测得的MS2噬菌体（MS2 phage）基因组RNA修饰谱与正交液相色谱-串联质谱（orthogonal LC-MS/MS）检测结果。结果显示二者对RNA修饰图谱的认知存在显著差异，这提示仅依靠纳米孔测序鉴定新型RNA修饰时需谨慎行事。