Transcriptomic Response of Adult Fruit Flies to Arsenite Exposure II. Transcriptomic Response of Adult Fruit Flies to Arsenite Exposure II

As part of our exploration into alternative model systems for toxicology, we exposed male and female adult D. melanogaster to varying concentratios of arsenite over the course of two days and profiled their response via RNA-seq Overall design: Male and female starved adult flies were exposed to zero (control), 0.25mM, or 1mM sodium arsenite in their food for the alotted time (0, 1, 2, 4, 8, 12, 24, 48h), before collection and proccessing for RNA-seq These data stem from an in-house re-run of experimental samples initially profiled in GSE235705, which was missing several male timepoints and had a high variance of read depth A few samples were re-run for increased depth; the concatenated fastq files are identified by their "S00" sample numbers raw fastq files are coded with the schema: "[NaAsO2 (soduium arsenite)]- [M/F (male or female) 0/1/2/4/8/24/48 (time of treatment in hours) A/B/C (0, 0.25, or 1mM arsenite, respectively)]-[1/2/3/4 (replicate number)]

本研究围绕毒理学替代模型体系展开探索，将成年雌雄黑腹果蝇（Drosophila melanogaster）暴露于不同浓度的亚砷酸盐环境中，处理时长总计两天，并通过RNA测序（RNA-seq）对其转录应答进行全景分析。
整体实验设计：将经饥饿处理的成年雌雄果蝇置于添加了0（对照组）、0.25mM、1mM亚砷酸钠的食物中，分别处理指定时长（0、1、2、4、8、12、24、48小时），随后收集样本并开展RNA-seq建库与测序。
本数据集源自对初始收录于GSE235705的实验样本的内部重测：原数据集存在部分雄性样本时间点缺失、测序读深方差偏高的问题。部分样本因需提升测序深度而进行了重测；合并后的FASTQ文件以"S00"样本编号标识。原始FASTQ文件的命名规则为："[亚砷酸钠（NaAsO₂）]-[M/F（雌雄）/处理时长（小时，取值为0、1、2、4、8、12、24、48）/A/B/C（分别对应0、0.25、1mM亚砷酸盐）]-[1/2/3/4（生物学重复编号）]"