Intra-MZ pair discordance and longitudinal variation of whole-genome scale DNA methylation in adults

In this study, we used Illumina Infinium HumanMethylation450 Beadchips to compare DNA methylation profiles in blood from 10 pairs of MZ twins and 8 individuals recruited at 0, 3, 6, and 9 months. MZ Group (Group A) contained 10 pairs of MZ twins ranging from 23 to 74 years old, including 8 female and 12 male subjects.Longitudinal study group (Group B) included a pair of MZ (male) twins and 6 unrelated individuals (3 male, 3 female), aged from 24 to 39. Except subject H, all participants in Longitudinal study group (Group B) were recalled every 3 months for 9 months (0, 3, 6, and 9 m). Subject H was studied only at 0, 6, and 9 months. Bisulphite converted DNA from the 60 samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip

本研究采用Illumina Infinium HumanMethylation450 BeadChip芯片，对10对同卵双胞胎（MZ, Monozygotic Twins）以及8名于0、3、6、9个月时间点入组的个体的血液DNA甲基化谱进行比较分析。同卵双胞胎组（A组）包含10对年龄介于23至74岁的同卵双胞胎，共计8名女性、12名男性受试者。纵向研究组（B组）纳入1对男性同卵双胞胎与6名无关个体（3男3女），年龄区间为24至39岁。除受试者H外，纵向研究组（B组）其余所有参与者均在9个月随访周期内每3个月接受一次回访，采样时间点分别为0、3、6、9个月；受试者H仅在0、6、9个月三个时间点接受检测。本研究对60份样本的亚硫酸氢盐转化DNA（Bisulphite converted DNA）进行了Illumina Infinium 450K人类甲基化芯片杂交。