mRNA expression profiles in far-infrared treated human endothelial precursor cells

High glucose impairs the angiogenic activities of late endothelial precursor cells (EPC). We found that far infrared (FIR) treatment restored partially the activity of late EPC. However, the mechanisms are unclear. We performed gene expression microarray analysis to assess the expression profiles of high glucose-treated late EPC with or without FIR treatment. Late EPCs isolated from peripheral blood were cultured in high glucose condition for 48 hours, and then treated with or without FIR radiation for 30 minutes. Total RNA were extracted and evaluated with the Affymetrix GeneChip Human U133 plus 2.0 arrays.

高葡萄糖浓度可损伤晚期内皮祖细胞（late endothelial precursor cells, EPC）的血管生成活性。本研究发现，远红外线（far infrared, FIR）处理能够部分恢复晚期EPC的该活性，但其具体作用机制尚不明确。为此，我们开展基因表达微阵列分析，以评估经高葡萄糖处理且伴或不伴FIR处理的晚期EPC的基因表达谱。实验中，研究人员从外周血中分离得到晚期EPC，将其置于高葡萄糖环境中培养48小时，随后分别接受或不接受30分钟的FIR辐照。之后提取总RNA，采用Affymetrix Human U133 Plus 2.0基因芯片进行检测分析。