Expression data from early Drosophila embryo

Translational control is critical for early Drosophila embryogenesis and is exerted mainly at the gene-specific level. To understand the post-transcriptional regulation during Drosophila early embryonic development, we used the sucrose polysomal gradient analyses and GeneChip analysis to illustrate the translation profile of individual mRNAs. A paper was published in Genome Biology 2007 under the tile "Global analyses of mRNA translational control during early Drosophila embryogenesis". Keywords: time course, fractionation In this study, we have fractionated embryo extracts from a series of early stages by sedimentation on sucrose density gradients and analyzed the RNA components of these fractions. Our analysis has focused on analyzing ribosomal density, generally and for individual transcripts, global translational activity during the first 10 hours after egg laying and coordination between transcription and translation regulation.

翻译调控对于果蝇（Drosophila）早期胚胎发生至关重要，且主要以基因特异性方式发挥作用。为阐明果蝇早期胚胎发育过程中的转录后调控机制，本研究采用蔗糖多聚核糖体梯度分析与基因芯片（GeneChip）分析技术，对单个信使RNA（mRNA）的翻译谱进行了表征。相关研究成果于2007年发表于《基因组生物学（Genome Biology）》期刊，论文标题为《果蝇早期胚胎发生过程中mRNA翻译调控的全局分析（Global analyses of mRNA translational control during early Drosophila embryogenesis）》。关键词：时间进程实验（time course）、分级分离（fractionation）。本研究通过蔗糖密度梯度沉降法，对一系列早期发育阶段的胚胎提取物进行分级分离，并分析了各分级组分中的RNA成分。本研究的分析重点涵盖：整体及单个转录本的核糖体密度、果蝇产卵后最初10小时内的全局翻译活性，以及转录调控与翻译调控之间的协同关系。