Molecular mapping of quantitative trait loci for agronomical traits in soybean under Asian soybean rust infection

Abstract Asian soybean rust (ASR) is one of the most serious diseases for soybean crops, and it can be responsible for severe yield reduction. Due to the low durability of vertical resistance, studies on horizontal resistance are important for breeding programs. The objective of this study was to identify quantitative trait loci (QTLs) related to ASR horizontal resistance. A genetic linkage map with 26 groups was generated for simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers from 163 recombinant inbred lines (RILs). We found 13 QTLs related to 12 traits, including eight directly related to plant resistance. Furthermore, two QTLs controlling more than one trait were observed: one was related to agronomical traits, and another was related to a similar region of the Rpp3 locus associated with the number of uredinia and sporulation levels. These results will be useful for breeding programs to generate cultivars resistant to ASR.【摘要】亚洲大豆锈病（Asian soybean rust, ASR）是危害大豆作物的最严重病害之一，可引发严重减产。由于垂直抗性的持效性较低，开展水平抗性相关研究对大豆育种项目具有重要意义。本研究旨在挖掘与ASR水平抗性相关的数量性状位点（quantitative trait loci, QTLs）。研究以163份重组自交系（recombinant inbred lines, RILs）为材料，开发了简单序列重复（simple sequence repeat, SSR）与单核苷酸多态性（single nucleotide polymorphism, SNP）标记，并构建了包含26个连锁群的遗传连锁图谱。本研究共鉴定得到13个与12个性状相关的QTLs，其中8个直接与植株抗病性相关。此外，还发现2个可调控多个性状的QTL：一个与农艺性状相关，另一个位于与夏孢子堆数量和产孢水平相关的Rpp3位点相似区域。上述研究结果可为培育抗ASR大豆品种提供理论支撑。