Electron microscopy of SARS-CoV particles - Dataset 11

The dataset contains transmission electron microscopy image stacks (tomograms) of ultrathin sections through extracellular SARS-CoV particles in Vero cell cultures. The dataset contains 10 image stacks of 1900 x 1900 pixel dimensions, which were recorded at 0.57 nm pixel size (12 bit). Image stacks were size calibrated and stored in 8 bit TIF format. Visualization can be done using ImageJ or Fiji. A PDF document describes the methods used for generation of the image files. The dataset was generated as dataset 11 for a comparative morphometric analysis of SARS-CoV and SARS-CoV-2. Further datasets which were used for the analysis are available in this repository (see dataset description document). Related publication: Laue M, Kauter A, Hoffmann T, Möller L, Michel J, Nitsche A. Morphometry of SARS-CoV and SARS-CoV-2 particles in ultrathin plastic sections of infected Vero cell cultures. Sci Rep. 2021 Feb 10;11(1):3515. doi: 10.1038/s41598-021-82852-7. PMID: 33568700; PMCID: PMC7876034.本数据集包含非洲绿猴肾细胞（Vero细胞）培养物中细胞外SARS冠状病毒（SARS-CoV）颗粒的超薄切片的透射电子显微镜图像栈（断层扫描图像，tomograms）。该数据集包含10组尺寸为1900×1900像素的图像栈，成像像素尺寸为0.57 nm，采集时采用12位深度。所有图像栈均经过尺寸校准，并以8位TIF格式存储。可使用ImageJ或Fiji软件进行图像可视化。另有一份PDF文档详细说明了该图像文件的生成方法。本数据集是为开展SARS冠状病毒（SARS-CoV）与SARS冠状病毒2型（SARS-CoV-2）的比较形态计量学分析所构建的数据集11。本分析所用的其余数据集可在本数据仓库中获取（详见数据集描述文档）。 相关发表文献：Laue M, Kauter A, Hoffmann T, Möller L, Michel J, Nitsche A. 感染Vero细胞培养物的超薄塑料切片中SARS-CoV与SARS-CoV-2病毒颗粒的形态计量学分析. 科学报告. 2021年2月10日;11(1):3515. DOI: 10.1038/s41598-021-82852-7. PMID: 33568700; PMCID: PMC7876034.