IRF5 regulates unique subset of genes in mouse dendritic cells during West Nile virus infection (BMM_RNA-Seq). IRF5 regulates unique subset of genes in mouse dendritic cells during West Nile virus infection (BMM_RNA-Seq)

Using integrative bioinformatics analyses, we identified new IRF5 primary target genes in mouse DCs in response to virus infection. This study provides novel insights into the distinct and unique innate immune and immune gene regulatory program directed by IRF5. Overall design: Total RNA-seq profiling of mouse bone marrow-derived macrophages infected with WNV and mock infection at 6h and 12h

本研究通过整合生物信息学分析，鉴定出病毒感染条件下小鼠树突状细胞（DCs）中干扰素调节因子5（IRF5）的新型初级靶基因。本研究为解析IRF5介导的独特先天免疫与免疫基因调控程序提供了全新视角。实验设计概况：对感染西尼罗病毒（WNV）及空白对照感染的小鼠骨髓源巨噬细胞，分别在感染后6小时和12小时开展总RNA测序（Total RNA-seq）转录组分析。