Development of Fe3O4/reduced graphene oxide nanocomposite-based dispersive solid-phase extraction for the quantification of five steroid hormones: supplementary materials

Aim: An accurate and sensitive analytical method was proposed to detect some steroid hormones
in biological samples. Materials & methods: An Fe3O4/reduced graphene oxide nanocomposite-based
dispersive solid-phase extraction was developed for the effective and simple preconcentration of steroid
hormones from human serum samples. Results & conclusion: The nanocomposite was firstly used as
adsorbent to simultaneously extract the selected hormones. Limit of detection values for the selected
hormones were calculated between 5.5 and 39.2 ng/kg (mass based). An artificial serum sample was used
to test the applicability and accuracy of the developedmethod; percentage recovery results obtained from
two different spiked concentrations were found to be in the range of 80.5–99.9%.

研究目的：提出一种准确且灵敏的分析方法，用于检测生物样品中的部分类固醇激素(steroid hormones)。
材料与方法：开发了一种基于四氧化三铁(Fe3O4)/还原氧化石墨烯(reduced graphene oxide)纳米复合材料(nanocomposite)的分散固相萃取(dispersive solid-phase extraction)方法，可从人体血清样品中高效、简便地预富集类固醇激素。
结果与结论：该纳米复合材料首次作为吸附剂，用于同时萃取所选目标类固醇激素。目标激素的检出限(limit of detection)值介于5.5~39.2 ng/kg（以质量计）。采用人工血清样品对所开发方法的适用性与准确性进行验证；两种不同加标浓度下得到的回收率结果处于80.5%~99.9%的范围内。