Mining-impacted water metagenome. terrestrial metagenome

Five different samples (one from an acid-mine drainage biofilm, one from an anaerobic brewery-waste digester and three samples from different sulfate-reducing bioreactors) were pooled, sequenced and assembled together. All samples were submitted to the sequencing facility in separate eppendorf tubes. At the sequencing facility, a library prep was performed and all samples were thereafter pooled (multiplexed) and sequenced together in the same HiSeq lane. After sequencing, the data was demultiplexed to distinguish between samples. Each assembly was however performed on the entire dataset. For Desulfobacteraceae bacterium RAAP-1: 100% of the reads came from bioreactor metagenome SAMN04151528. For Acidimicrobiaceae bacterium RAAP-2 (Cluster 30) read distribution used for assembly: 15% reads came from bioreactor metagenome SAMN04151528 and 85% came from acid mine drainage metagenome SAMN04151554. For Acidimicrobiaceae bacterium RAAP-3 (Cluster_164): read distribution used for assembly: 4.5% reads came from bioreactor metagenome SAMN04151528 and 95.5% came from acid mine drainage metagenome SAMN04151554.

本研究共涉及5份环境样本：1份取自酸性矿山排水生物膜，1份取自厌氧啤酒废水消化器，另外3份来自不同的硫酸盐还原生物反应器。上述样本被混合后进行测序与组装。所有样本均以独立的Eppendorf离心管分装后提交至测序服务机构。在测序服务机构完成文库制备后，将所有样本进行多重标记混样，并在同一HiSeq测序泳道中完成测序。测序完成后，对原始测序数据进行解多重标记操作，以区分不同来源的样本，但后续所有基因组组装均基于全部测序数据集完成。针对脱硫杆菌科（Desulfobacteraceae）细菌RAAP-1：用于组装的全部测序读段（reads）均来自生物反应器宏基因组SAMN04151528。针对酸微菌科（Acidimicrobiaceae）细菌RAAP-2（聚类簇30），组装所用测序读段的来源分布为：15%来自生物反应器宏基因组SAMN04151528，85%来自酸性矿山排水宏基因组SAMN04151554。针对酸微菌科（Acidimicrobiaceae）细菌RAAP-3（聚类簇164），组装所用测序读段的来源分布为：4.5%来自生物反应器宏基因组SAMN04151528，95.5%来自酸性矿山排水宏基因组SAMN04151554。