SH1043918.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、传播信息并处理基于DNA的物种假说（Species Hypotheses，SH）。国际核酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，均通过应用一组动态距离阈值（0.5%~3.0%）聚类至近似物种水平。所有物种假说均会被赋予一个采用数字对象标识符（Digital Object Identifier，DOI）格式的唯一稳定名称，其分类学与生态学注释将通过分布式网络第三方注释工作完成验证。系统会结合该物种假说下所有序列的鉴定结果，尽可能为其匹配对应的分类单元名称及其分类层级（如门、纲、目等）。研究人员将选取一条自动或人工指定的序列作为该物种假说的代表序列。此类代表序列已公开发布（https://unite.ut.ee/repository.php），可供科研社区用于本地序列相似性检索、新一代测序分析流程等科研场景。随着公共真菌ITS序列数据量的持续增长，该系统及其配套数据库会自动进行更新。