To identify potential ubiquitin ligases that regulate BIK1 homeostasis. To identify potential ubiquitin ligases that regulate BIK1 homeostasis

To identify potential ubiquitin ligases that regulate BIK1 homeostasis,A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. We using an optimized data analysis workflow, Overall design: RNA isolated from seedlings of 12-day old wild type arabidopsis plants(Col-0) treated with H2O and flg22,and with 3 repeats of each sample

为筛选调控BIK1稳态的潜在泛素连接酶（ubiquitin ligase），本实验每份样品取3 µg总RNA作为RNA样品制备的起始材料。参照制造商推荐的操作流程，使用NEBNext® Ultra™ 适配Illumina®测序平台的RNA文库制备试剂盒（美国NEB公司）构建测序文库，并添加索引编码以将测序序列归属至对应样品。我们采用了优化的数据分析流程。
实验整体设计：以生长12天的野生型拟南芥（Arabidopsis thaliana，Col-0生态型）幼苗为材料，分别经H₂O与flg22处理，每份样品设置3次生物学重复，随后提取总RNA。