SH1410910.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、交流以及处理基于DNA的物种假说（Species Hypotheses，以下简称SH）。国际核酸序列数据库收录的全部真菌内转录间隔区（Internal Transcribed Spacer，以下简称ITS）序列，均通过应用一系列动态距离阈值（0.5%~3.0%）聚类至近似物种层级。所有SH均被赋予以数字对象唯一标识符（Digital Object Identifier，以下简称DOI）为格式的唯一稳定标识，其分类学与生态学注释信息将通过分布式、基于网页的第三方注释工作完成核验。系统将结合每条SH内所有序列的鉴定结果，尽可能将其关联至对应的分类单元名称及其所属分类层级（如门、纲、目等）。为每条此类SH选取一条经自动或手动指定的序列作为代表序列。上述代表序列已发布于https://unite.ut.ee/repository.php，可供科学界用于本地序列相似性搜索、下一代测序分析流程等研究场景。随着公共真菌ITS序列数量的持续增长，该系统及其配套数据将自动完成更新。