Additional file 30 of Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma

Additional file 30: Table S8. HOGA1 inactivation promotes pancreatic cancer growth through activating LARP7-CDK1 pathway. Table S8A Quantified western blot results of 12 pairs of samples. Table S8B Proliferation of PANC-1 and BxPC-3 cells associated with HOGA1 knocked down. Table S8C Proliferation of PANC-1 and BxPC-3 cells associated with HOGA1 overexpressed. Table S8D Proliferation of PANC-1 and BxPC-3 cells associated with metabolic enzyme treatment. Table S8E A list of 773 different proteins detected in PANC-1 cells. Table S8F GO term category of HOGA1 interacting proteins in cultured PANC-1 cells. Table S8G LARP7 mRNA levels in PANC-1 cells with various treatments. Table S8H The percentage of G2/M phase cells in PANC-1 cells. Table S8I Tumor weight of indicated PANC-1 cells subcutaneously injected into nude mice. Table S8J MS matrix of protein groups and their relative intensities in WT, HOGA1 KD and HOGA1 OE groups. Table S8K GO term category of proteins upregulated in HOGA1 KD group and downregulated in HOGA1 OE group.

附加文件30：表S8。HOGA1失活可通过激活LARP7-CDK1通路促进胰腺癌生长。表S8A：12对样本的定量蛋白质印迹（western blot）检测结果；表S8B：敲低HOGA1后PANC-1与BxPC-3细胞的增殖活性；表S8C：过表达HOGA1后PANC-1与BxPC-3细胞的增殖活性；表S8D：经代谢酶处理后PANC-1与BxPC-3细胞的增殖活性；表S8E：PANC-1细胞中检出的773种差异蛋白名录；表S8F：培养状态下PANC-1细胞中HOGA1互作蛋白的基因本体论（Gene Ontology，GO）功能分类；表S8G：经不同处理的PANC-1细胞内LARP7的mRNA水平；表S8H：PANC-1细胞的G2/M期细胞占比；表S8I：皮下接种裸鼠的指定PANC-1细胞的肿瘤重量；表S8J：野生型（wild type，WT）、HOGA1敲低组与HOGA1过表达组的蛋白组质谱（Mass Spectrometry，MS）矩阵及其相对强度；表S8K：HOGA1敲低组上调、HOGA1过表达组下调的蛋白的GO功能分类。