Details of primary antibodies used for immunohistochemistry.

αSMA, α-smooth muscle actin; PAR1, proteinase activated receptor 1; SCMW, sodium citrate buffer (2.94 g/L) microwave,
*used for total hepatic macrophage counting with light microscopy.
**used for fluorescence immunodetection of macrophages in conjunction with in situ hybridisation for Y chromosome.
§a kind gift from Dr Eleanor Mackie, Melbourne, Australia.

αSMA，即α-平滑肌肌动蛋白（α-smooth muscle actin）；PAR1，即蛋白酶激活受体1（proteinase activated receptor 1）；SCMW，即2.94 g/L柠檬酸钠缓冲液微波处理法，
* 用于光学显微镜下总肝巨噬细胞计数。
** 用于巨噬细胞的荧光免疫检测，并结合Y染色体原位杂交实验。
§ 由澳大利亚墨尔本的埃莉诺·麦基博士惠赠。