Target Capture Sequencing of SARS-CoV-2 Genomes Using the ONETest Coronaviruses Plus

We introduce a target capture next-generation sequencing methodology, the ONETest Coronaviruses Plus (Fusion Genomics Corp.), to sequence SARS-CoV-2 genomes and select loci of other respiratory viruses. We applied the ONETest on 70 respiratory samples (collected in Florida, USA between May and July, 2020), in which SARS-CoV-2 had been detected by a qualitative PCR assay. For 48 of the samples, we also applied the ARTIC protocol.本研究介绍了一款靶向捕获型下一代测序技术——ONETest冠状病毒Plus（Fusion Genomics Corp.），可用于对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）基因组进行测序，并靶向捕获其他呼吸道病毒的基因位点。我们将该技术应用于70份呼吸道样本（2020年5月至7月采集自美国佛罗里达州），所有样本均已通过定性PCR检测确认携带SARS-CoV-2。其中48份样本同时采用了ARTIC测序方案开展实验。